ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 26 kDa band observed in human cerebellum lysates (calculated MW of 28.5 kDa according to NP_075012.1 and NP_075014.1). Recommended concentration: 0.3-1 μg/mL.
SMN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SMN1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Antigen: SMN1 / SMN2