ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 110 kDa band observed in human pancreas lysates (calculated MW of 93.9 kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approximately 95 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
Smek1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Smek1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.