ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 120 kDa and 50 kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1 μg/mL. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western Blot observations (J Biol Chem 1995 Mar 3;270 (9) :4575-87).
SMARCA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SMARCA3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.