ELISA: Antibody detection limit dilution 1:64,000. Immunohistochemistry: In paraffin embedded human spinal cord shows staining of the cellular membrane in the bundles of sensory fibres. Recommended concentration, 4-8 μg/mL.
SLIT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SLIT2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.