ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 40 kDa band observed in mouse eye and rat skin lysates (calculated MW of 54.9 according to human NP_995322.1, of 55.0 kDa according to mouse NP_778199.2 and of 38.1 kDa according to rat NP_001101239.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ginger et al, J Biol Chem. 2008 Feb 29;283 (9) :5486-95; PMID: 18166528). Recommended concentration: 1-3 μg/mL.
SLC24A5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SLC24A5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Mouse, Rat