ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 28 kDa band observed in human liver lysates (calculated MW of 28.6 kDa according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approximately 35 kDa and 48 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
SIRT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SIRT3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.