ELISA: Antibody detection limit dilution 1:32,000. Approximately 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112 kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272 (44) :28081-8; PMID: 9346962). Recommended concentration: 1-3 μg/mL. an additional band of 55-60 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this antibody is used for other assays than Western Blot.
SIPA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SIPA1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.