ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human lung and kidney lysates (predicted MW of 71 kDa according to NP_061830). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3 μg/mL.
SH3BP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SH3BP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.