ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 16 kDa band observed in human thymus lysates (calculated MW of 14.2x kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approximately 16 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
SH2D1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SH2D1A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.