ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 50 kDa band observed in lysates of human EBV immortalised lymphoblastoids, T cell MOLT4 and monocytic cell line U937 (calculated MW of 51.8 kDa according to NP_000859.1). Recommended concentration: 1-3 μg/mL.
Serotonin receptor 2C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Serotonin receptor 2C antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.