ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 48 kDa band observed in human brain lysates (calculated MW of 43.6 kDa according to NP_000854.1). Recommended concentration: 1-3 μg/mL.
Serotonin receptor 1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Serotonin receptor 1B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.