ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul;26 (13) :4895-910; PMID: 16782878). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.
SBP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SBP2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.