ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 13 kDa band observed in human placenta lysates (calculated MW of 11.7 kDa according to NP_002952.1and NP_062427.1). Recommended concentration: 0.03-0.1 μg/mL.
S100A4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
S100A4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Antigen: S100 A4