ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 48 kDa band observed in human brain lysates (calculated MW of 50.9 kDa according to NP_008848). Recommended concentration: 0.3-2 μg/mL. Some bands of unknown identity were also consistently observed around 37 kDa . These bands were successfully blocked by incubation with the immunizing peptide.
RXR gamma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RXR gamma antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.