ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in rat retina lysates (calculated MW of 60.9 kDa according to human NP_000320.1 and 61.0 kDa according to rat NP_446014.1). Recommended concentration: 0.5-1.5 μg/mL.
RPE65 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RPE65 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.