ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 150 kDa band observed in human brain (cerebellum and hippocampus) lysates (calculated MW of 148 kDa according to NP_071765.2). Recommended concentration: 0.5-1 μg/mL.
Robo3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Robo3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.