ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 37-38 kDa band observed in lysates of cell lines of NIH3T3 and K562 (calculated MW of 37.7 kDa according to NP_009143.1). Recommended concentration: 0.3-1 μg/mL.
RNF2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RNF2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.