ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 17-18 kDa and 25 kDa in human liver lysates after 0.5 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in neuronal bodies. Recommended concentration, 3-6 μg/mL.
Ribosomal Protein L22 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Ribosomal Protein L22 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.