ELISA: Antibody detection limit dilution 1:32000. Western Blot: No signal obtained yet but low background observed in human testis lysate at up to 1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong staining of cytoplasm in DCT. Recommended concentration, 3-5 μg/mL.
RFI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RFI antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.