ELISA: Antibody detection limit dilution 1:8,000. Western Blot: experiments gave bands at approximately 95 kDa, 75 kDa and 45 kDa in A549 lung carcinoma lysate after 0.3 μg/mL antibody staining. The detected bands were successfully blocked by incubation with the immunizing peptide.
RASIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RASIP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.