ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 μg/mL.
Rabenosyn 5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Rabenosyn 5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.