ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in mouse liver lysates (calculated MW of 130 kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0.03-0.1 μg/mL.
Pyruvate carboxylase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pyruvate carboxylase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.