ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 28 kDa band in human brain, duodenum and heart lysates after 1 μg/mL antibody staining. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.
PTCH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PTCH antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.