ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 60-65 kDa band observed in Jurkat lysate (predicted MW of 64-, 63- and 61- kDa according to NP_002810, NP_114367 and NP_114368). Recommended for use at 1 μg/mL.
PTB antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PTB antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.