ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 45 kDa band in human brain, lymph node, spleen and testis lysates after 0.3 μg/mL antibody staining.7 kDa according to NP_002717.3. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney shows distinct staining patterns in the cytoplam of PCT. Recommended concentration, 3-6 μg/mL.
Prolyl Endopeptidase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Prolyl Endopeptidase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.