ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 85 kDa and 25 kDa in human brain lysates after 1 μg/mL antibody staining.0 kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry In paraffin embedded cerebellum shows mitochondrial staining in smaller neurons. Recommended concentration, 3-6 μg/mL.
PRODH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PRODH antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.