ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 17 kDa band in human lung lysates after 1 μg/mL antibody staining.6 kDa according to NP_006141.2. The 17 kDa band was successfully blocked by incubation with the immunizing peptide.
PRICKLE3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PRICKLE3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.