ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 55 kDa and 40 kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 μg/mL. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271 (36) :22081-9).
PPP2R5E antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PPP2R5E antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.