ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 26 kDa band observed in lysates of mouse pituitary cell line NIH3T3 (calculated MW of 29.4 kDa according to NP_000930.1 and NP_001030333.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human adrenal gland shows strong cytoplasm staining in select cells in the medulla. Recommended concentration, 2-4 μg/mL.
POMC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
POMC antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse