ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Experiments gave a band at approximately 40 kDa in human liver and HepG2 extracts at 1 μg/mL. Immunohistochemistry: In paraffin embedded human small intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10 μg/mL.
POLL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
POLL antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.