ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 45 kDa band observed in human heart and PBMC lysates (predicted MW of 40 kDa according to NP_002655). Recommended for use at 0.05-1 μg/mL.
Pleckstrin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pleckstrin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.