ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 40 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 31.8 kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9;282 (45) :33192-200.; PMID: 17848564). Recommended concentration: 0.3-1 μg/mL.
PITX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PITX3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.