ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 50 kDa band in human heart lysates after 0.1 μg/mL antibody staining. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebral cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10 μg/mL.
PITPNM3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PITPNM3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.