ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 55 kDa band observed in human frontal cortex lysates (calculated MW of 50.5 kDa according to NP_065132.1). Recommended concentration: 0.05-0.2 μg/mL.
PIST antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PIST antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.