ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 33 kDa band observed in human heart lysate (calculated MW of 32.1 kDa according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approximately 35 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 μg/mL.
Pirin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pirin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.