ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 17 kDa band observed in rat brain lysates (calculated MW of 18.2 kDa according to human NP_006212.1 and 18.2 kDa according to mouse NP_075860). Recommended concentration: 0.2-0.6 μg/mL.
PIN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PIN1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.