ELISA: Antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/mL). Immunohistochemistry: In paraffin embedded human cerebral cortex shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3 μg/mL.
PIK3CA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PIK3CA antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.