ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 35 kDa band observed in human kidney lysates (calculated MW of 29.8 kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approximately 35 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
PI31 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PI31 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.