ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 85 kDa band observed in mouse brain lysates (calculated MW of 83.3 kDa according to human NP_002591.2 and 82.1 kDa according to mouse NP_062814.2). In transfected HEK293 transiently expressing human PDE4B a band of approximately 85 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 μg/mL.
Phosphodiesterase 4B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
pH ospH odiesterase 4B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.