ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 140 kDa band observed in human brain (amygdala, hippocampus, substantia nigra) lysates (calculated MW of 134 kDa according to NP_055835.2). Recommended concentration: 0.1-0.3 μg/mL.
PHLPP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
pH LPP2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.