ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 70+80+90 kDa bands observed in A549 lysate . In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approximately 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from Approximately 55 kDa to 80 kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1 kDa according to PDE4D1 (NP_001098101.1), 76.5 kDa according to PDE4D2 (NP_006194.2) and 84.7 kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3 μg/mL.
PDE4D antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PDE4D antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse