ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 70 kDa band observed in human kidney lysates (calculated MW of 69.2 kDa according to NP_002582.2). Recommended concentration: 0.5-1.5 μg/mL.
PCK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PCK1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.