ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 40 kDa band observed in HeLa lysate (calculated MW of 41.4 kDa according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approximately 45 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
PCBP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PCBP4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.