ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa + 40 kDa bands observed in HEK293 lysate (calculated MW of 48.2 kDa according to PAX8A, NP_003457.1 and 41.7 kDa according to PAX8B, NP_039245.1). Recommended concentration: 0.5-1.5 μg/mL.
PAX8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PAX8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.