ELISA: Antibody detection limit dilution >1:32,000. Western Blot: an approximately 40 kDa band observed in Human Liver lysates. Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cells around follicles. Recommended concentration, 1-2 μg/mL. Does not stain mouse tissues
PAX8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PAX8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.