ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 74 kDa band observed in human spleen and human liver lysates (calculated MW of 74.1 kDa according to NP_036519). Recommended concentration: 0.3-2 μg/mL.
PAD4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PAD4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.