ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3 kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
ORC3L antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ORC3L antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.