ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in human thyroid gland lysates (calculated MW of 64.5 kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3 μg/mL.
Nur77 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Nur77 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.