ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 30 kDa band in human testis lysates after 0.5 μg/mL antibody staining. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.
NUP62 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NUP62 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.