ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 60 kDa band observed in mouse brain lysates (calculated MW of 54.2 kDa according to NP_031389.3). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human spleen shows nuclear staining in select splenocytes. Recommended concentration, 3-6 μg/mL.
NONO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NONO antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse